Product Description |
Recombinant Mouse tPA-Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser33-Gln559, which consists of two chains: chain A (Ser33-Arg308) and chain B (Ile309-Gln559). |
Product Background |
Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the predominant route for enhancing cell survival is via an mTORdependent mechanism. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
tPA |
Synonyms |
t-PA; tPA; Alteplase; Reteplase; PLAT; |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ser33-Gln559 |
Molecular Weight |
The protein has a predicted MW of 60.57 kDa. It migrates to 13-15 kDa, 30-34 kDa and 35-40 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Mouse tPA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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