Mouse tPA-Protein (TPA-MM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Exact Sequence: Ser33-Gln559

Catalog: TPA-MM101

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Product Description Recombinant Mouse tPA-Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser33-Gln559, which consists of two chains: chain A (Ser33-Arg308) and chain B (Ile309-Gln559).
Product Category Recombinant Protein
Target tPA
Gene/Protein ID P11214
Species Mouse
Exact Sequence Ser33-Gln559
Molecular Weight The protein has a predicted MW of 60.57 kDa. It migrates to 13-15 kDa (chain A and chain B fragment), 30-34 kDa and 35-40 kDa based on Tris-Bis PAGE result.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is > 400 pmol/min/ug.
Mouse tPA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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