Name: Mouse tPA Protein (TPA-MM101)
Brand: KactusBio
SKU: TPA-MM101-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is > 400 pmol/min/ug.
Amino Acid Range: Ser33-Gln559

Catalog #TPA-MM101

Size

100UG

500UG

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Mouse tPA Protein (TPA-MM101) Summary

Product Description	Recombinant Mouse tPA-Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser33-Gln559, which consists of two chains: chain A (Ser33-Arg308) and chain B (Ile309-Gln559).
Product Background	Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the predominant route for enhancing cell survival is via an mTORdependent mechanism.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Mouse tPA Protein (TPA-MM101) Specifications/Details

Protein	tPA
Synonyms	t-PA; tPA; Alteplase; Reteplase; PLAT;
Accession	P11214
Species	Mouse
Biotinylated	no
Amino Acid Range	Ser33-Gln559
Molecular Weight	The protein has a predicted MW of 60.57 kDa. It migrates to 13-15 kDa, 30-34 kDa and 35-40 kDa based on Bis-Tris PAGE result.
Activity	Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is > 400 pmol/min/ug.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Mouse tPA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Other Available Proteins:

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