Mouse tPA-Protein (TPA-MM201)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Ser33-Gln559
Catalog #TPA-MM201
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Product Description
 Recombinant Mouse tPA-Protein is expressed from HEK293 with hFc tag at the N-terminus.It contains Ser33-Gln559, which consists of two chains: chain A (Ser36-Arg308) and chain B (Ile309-Gln559).
Product Background
 Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the predominant route for enhancing cell survival is via an mTORdependent mechanism.
Product Category
 Recombinant Protein
Target
 tPA
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Ser33-Gln559
Molecular Weight
 The protein has a predicted MW of 85.00 kDa. The protein is activated by thermolysin and cleaved to a 2-chain protease, it migrates to 35-40 kDa (Chain A) and 30-35 kDa (Chain B) based on Bis-Tris PAGE result.
Product Tag
 N-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse tPA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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