Regular price
$417.00
Order by 4pm ET for delivery on:
Mouse Transferrin R/CD71 Protein, Ultra Low Endotoxin (TFR-MM101-UL) Summary
Product Description |
Recombinant Mouse Transferrin R/CD71 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Cys89-Phe763. |
Product Background |
The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Mouse Transferrin R/CD71 Protein, Ultra Low Endotoxin (TFR-MM101-UL) Specifications/Details
Protein |
Transferrin R/CD71 |
Synonyms |
TR; TfR1; CD71; sTfR; T9; p90; TFRC; TFR |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Cys89-Phe763 |
Molecular Weight |
The protein has a predicted MW of 76.99 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse Transferrin R, His Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin, His Avi Tag with the EC50 of 14.5ng/ml determined by ELISA. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Mouse Transferrin R, His Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin, His Avi Tag with the EC50 of 14.5ng/ml determined by ELISA. |
|
|
Mouse Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. |
|
|
The purity of Mouse Transferrin R is greater than 95% and the molecular weight of this protein is around 150-180 kDa as determined by SEC-MALS. |
|
|
The purity of Mouse Transferrin R is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
