Non-biotinylated Mouse uPAR/PLAUR Protein (PAR-MM401) Summary
Product Description |
Recombinant Mouse uPAR/PLAUR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu24-Gly298. |
Product Background |
The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Non-biotinylated Mouse uPAR/PLAUR Protein (PAR-MM401) Specifications/Details
Protein |
uPAR/PLAUR |
Synonyms |
U-PAR; uPAR; CD87; PLAUR; MO3; UPAR |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Leu24-Gly298 |
Molecular Weight |
The protein has a predicted MW of 32.92 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 5.87 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Mouse uPAR, His Tag immobilized on CM5 Chip can bind Human PLAU, His Tag with an affinity constant of 5.87 nM as determined in SPR assay (Biacore T200). |
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Mouse uPAR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse uPAR is greater than 95% as determined by SEC-HPLC. |
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