Mouse uPAR/PLAUR isoform 1 Protein (PAR-MM401)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 5.87 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Leu24-Gly298

Catalog: PAR-MM401

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Product Description	Recombinant Mouse uPAR/PLAUR isoform 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu24-Gly298.
Product Background	The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.
Product Category	Recombinant Protein

Target	uPAR/PLAUR isoform 1
Gene/Protein ID	P35456-1
Species	Mouse
Exact Sequence	Leu24-Gly298
Molecular Weight	The protein has a predicted MW of 32.92 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 5.87 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse uPAR isoform 1, His Tag immobilized on CM5 Chip can bind Human PLAU, His Tag with an affinity constant of 5.87 nM as determined in SPR assay (Biacore T200).
	The purity of Mouse uPAR isoform 1 is greater than 95% as determined by SEC-HPLC.
	Mouse uPAR isoform 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.