Mouse VCAM-1/CD106 Protein (VAM-MM106)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Phe25-Glu698

Catalog #VAM-MM106

Price

$346⁵⁰

Size

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Mouse VCAM-1/CD106 Protein (VAM-MM106) Summary

Product Description

Recombinant Mouse VCAM-1/CD106 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Glu698.

Product Background

Tumor necrosis factor alpha (TNFα) is a pro-inflammatory cytokine that triggers the expression of inflammatory molecules, including other cytokines and cell adhesion molecules. TNFα induces the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 was originally identified as a cell adhesion molecule that helps regulate inflammation-associated vascular adhesion and the transendothelial migration of leukocytes, such as macrophages and T cells.

Mouse VCAM-1/CD106 Protein (VAM-MM106) Specifications/Details

Protein	VCAM-1/CD106
Synonyms	V-CAM 1; VCAM-1; INCAM-100; CD106; DKFZp779G2333; MGC99561;VCAM1; INCAM100
Accession	NP_035823.3
Species	Mouse
Biotinylated	no
Amino Acid Range	Phe25-Glu698
Molecular Weight	The protein has a predicted MW of 75.48 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Mouse VCAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse VCAM-1 is greater than 95% as determined by SEC-HPLC.