Name: Mouse VEGF R3/FLT4 Protein (VGF-MM2R3)
Brand: KactusBio
SKU: VGF-MM2R3-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse VEGF R3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF-C, His Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test). The affinity constant of 12.93 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Tyr25-Glu775

Catalog #VGF-MM2R3

Size

100UG

500UG

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Mouse VEGF R3/FLT4 Protein (VGF-MM2R3) Summary

Product Description	Recombinant Mouse VEGF R3/FLT4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Tyr25-Glu775.
Product Background	Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Mouse VEGF R3/FLT4 Protein (VGF-MM2R3) Specifications/Details

Protein	VEGF R3/FLT4
Synonyms	VEGFR-3; FLT-4; LMPH1A; PCLFLT41; VEGFR3; FLT-41; PCL
Accession	P35917
Species	Mouse
Biotinylated	no
Amino Acid Range	Tyr25-Glu775
Molecular Weight	The protein has a predicted MW of 111.69 kDa. Due to glycosylation, the protein migrates to 115-160 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse VEGF R3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF-C, His Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test). The affinity constant of 12.93 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse VEGF R3, hFc Tag captured on CM5 Chip via Protein A can bind Human VEGF-C, His Tag with an affinity constant of 12.93 pM as determined in SPR assay (Biacore T200).
	Immobilized Mouse VEGF R3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF-C, His Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test).
	Mouse VEGF R3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse VEGF R3 is greater than 95% as determined by SEC-HPLC.