Mouse VEGF R3/FLT4 Protein (VGF-MM2R3)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse VEGF R3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF-C, His Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test). The affinity constant of 12.93 pM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Tyr25-Glu775

Catalog: VGF-MM2R3

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Product Description Recombinant Mouse VEGF R3/FLT4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Tyr25-Glu775.

Product Background

Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.

Product Category

Recombinant Protein

Target

VEGF R3/FLT4
Accession

Species

Mouse

Amino Acid Range

Tyr25-Glu775
Molecular Weight The protein has a predicted MW of 111.69 kDa. Due to glycosylation, the protein migrates to 115-160 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse VEGF R3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF-C, His Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test). The affinity constant of 12.93 pM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse VEGF R3, hFc Tag captured on CM5 Chip via Protein A can bind Human VEGF-C, His Tag with an affinity constant of 12.93 pM as determined in SPR assay (Biacore T200).
Immobilized Mouse VEGF R3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF-C, His Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test).
The purity of Mouse VEGF R3 is greater than 95% as determined by SEC-HPLC.
Mouse VEGF R3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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