Product Description |
Recombinant Mouse VEGF R3/FLT4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Tyr25-Glu775. |
Product Background |
Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery. |
Product Category |
Recombinant Protein |
Target |
VEGF R3/FLT4 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Tyr25-Glu775 |
Molecular Weight |
The protein has a predicted MW of 111.69 kDa. Due to glycosylation, the protein migrates to 115-160 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse VEGF R3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF-C, His Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test). The affinity constant of 12.93 pM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse VEGF R3, hFc Tag captured on CM5 Chip via Protein A can bind Human VEGF-C, His Tag with an affinity constant of 12.93 pM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Mouse VEGF R3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF-C, His Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test). |
|
|
Mouse VEGF R3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse VEGF R3 is greater than 95% as determined by SEC-HPLC. |
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