Regular price
$297.00
Order by 4pm ET for delivery on:
Non-biotinylated Human 4-1BB/TNFRSF9 Protein, Ultra Low Endotoxin (BB4-HM541-UL) Summary
Product Description |
Recombinant Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Leu24-Gln186. |
Product Background |
4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Non-biotinylated Human 4-1BB/TNFRSF9 Protein, Ultra Low Endotoxin (BB4-HM541-UL) Specifications/Details
Protein |
4-1BB/TNFRSF9 |
Synonyms |
CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu24-Gln186 |
Molecular Weight |
The protein has a predicted MW of 45.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1)-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human 4-1BB, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand (Trimer), hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. |
|
|
Human 4-1BB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Loaded Non-biotinylated Human 4-1BB, hFc-Avi Tag on ProA-Biosensor can bind Human 4-1BB Ligand (Trimer), His Tag with an affinity constant of 0.18 nM as determined in BLI assay . |
|
|
The purity of Human 4-1BB is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
