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Non-biotinylated Human CD160 Protein, Ultra Low Endotoxin (CD1-HM460-UL) Summary
Product Description |
Recombinant Human CD160 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Leu158. |
Product Background |
CD160 (also Natural killer cell receptor BY55) is a 27 ‑ 30 kDa member of the Ig superfamily. In human, it is expressed principally on nonmyeloid hematopoietic cells. CD160 antigenis a receptor on immune cells capable to deliver stimulatory or inhibitory signals that regulate cell activation and differentiation. Exists as a GPI-anchored and as a transmembrane form, each likely initiating distinct signaling pathways via phosphoinositol 3-kinase in activated NK cells and via LCK and CD247/CD3 zeta chain in activated T cells. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Non-biotinylated Human CD160 Protein, Ultra Low Endotoxin (CD1-HM460-UL) Specifications/Details
Protein |
CD160 |
Synonyms |
CD160;-BY55; BY55FLJ46513; NK1; NK28 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Leu158 |
Molecular Weight |
The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 27-33 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.44 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human HVEM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD160, His Tag with an affinity constant of 1.44 uM as determined in SPR assay (Biacore T200). |
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Human CD160 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human HVEM, hFc Tag on ProA-Biosensor can bind Human CD160, His Tag with an affinity constant of 0.95 µM as determined in BLI assay . |
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The purity of Human CD160 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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