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Non-biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein, Ultra Low Endotoxin (CD8-HM428-UL) Summary
Product Description |
Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152. |
Product Background |
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Non-biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein, Ultra Low Endotoxin (CD8-HM428-UL) Specifications/Details
Protein |
CD28 |
Synonyms |
CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290 |
Accession |
|
Species |
Human/Cynomolgus/Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Asn19-Pro152 |
Molecular Weight |
The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 23.9 ng/ml determined by ELISA (QC Test). The affinity constant of 43.61 uM as determined in SPR assay (Biacore T200). |
Product Tag |
N-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 43.61 uM as determined in SPR assay (Biacore T200). |
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Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15 nM as determined in a SPR assay (Biacore T200). |
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Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 23.9 ng/ml determined by ELISA (QC Test). |
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Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human/Cynomolgus/Rhesus macaque CD28 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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