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Non-biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein, Ultra Low Endotoxin (FCR-HM42B-UL) Summary
Product Description |
Recombinant Human Fc gamma RIIIB/CD16b (NA2) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly17-Ser200(NA2). The CD16b NA1 differ with the CD16b NA2 in AA36, 65, 82, and 106.The CD16b NA1 form carries R36, N65, D82, and V106, while the CD16b NA2 form carries S36,S65, N82, and I106. |
Product Background |
Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Non-biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein, Ultra Low Endotoxin (FCR-HM42B-UL) Specifications/Details
Protein |
Fc gamma RIIIB/CD16b |
Synonyms |
CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10; FcγRIIIB; Fc γRIIIB; Fcγ RIIIB; Fc γ RIIIB |
Accession |
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Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly17-Ser200(NA2) |
Molecular Weight |
The protein has a predicted MW of 23.7 kDa. Due to glycosylation, the protein migrates to 47-53 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 2.33 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human Fc gamma RIIIB (NA2), His Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 2.33 uM as determined in SPR assay (Biacore T200). |
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Human Fc gamma RIIIB (NA2) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human Fc gamma RIIIB (NA2), His Tag on Anti-His-Biosensor, can bind Rituximab, hFc Tag with an affinity constant of 2.87 µM as determined in BLI assay (Gator). |
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The purity of Human Fc gamma RIIIB (NA2) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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