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Non-biotinylated Human IL-4 Protein, Ultra Low Endotoxin (IL4-HM401-UL) Summary
Product Description |
Recombinant Human IL-4 is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His25-Ser153. |
Product Background |
Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4/IL-13 family. Interleukin-4/IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, and this cytokine is a ligand for interleukin 4 receptor. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Non-biotinylated Human IL-4 Protein, Ultra Low Endotoxin (IL4-HM401-UL) Specifications/Details
Protein |
IL-4 |
Synonyms |
IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
His25-Ser153 |
Molecular Weight |
The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-4 at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag (Cat. ILA-HM24R) with the EC50 of 4.2 ng/ml determined by ELISA (QC Test). |
Product Tag |
C-Avi-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human IL-4 at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag (Cat. ILA-HM24R) with the EC50 of 4.2 ng/ml determined by ELISA (QC Test). |
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Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is <0.2 ng/mL. |
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Loaded Human IL-4, His Tag on Anti-His-Biosensor can bind Human IL-4 R alpha, hFc Tag (Cat. ILA-HM24R) with an affinity constant of 0.54 nM as determined in BLI assay . |
Other Available Proteins:
Finding available proteins...
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