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Non-biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, Ultra Low Endotoxin (ITG-HM4V5-UL) Summary
Product Description |
Recombinant Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGAV.It contains Phe31-Val992(ITGAV) acidic tail and Gly24-Asn719(ITGB5) basic tail. |
Product Background |
Integrin ITGAV & ITGB5 is a type of integrin that binds to matrix macromolecules and proteinases and thereby stimulates angiogenesis. However, it inhibits angiogenesis. It consists of two components, integrin alpha V and integrin beta 5. Integrin ITGAV & ITGB5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.(Microbial infection)- Integrin ITGAV & ITGB5 acts as a receptor for adenovirus type C. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Non-biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, Ultra Low Endotoxin (ITG-HM4V5-UL) Specifications/Details
Protein |
Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer |
Synonyms |
αVβ5; Integrin alpha V beta 5; beta5; alphaV; ITGAV&ITGB5;Integrin alpha V β 5; β5 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe31-Val992(ITGAV) acidic tail & Gly24-Asn719(ITGB5) basic tail |
Molecular Weight |
The protein has a predicted MW of 114 kDa (ITGAV) & 81.4 kDa (ITGB5). Due to glycosylation,the protein migrates to 85-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ITGAV&ITGB5, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human ITGAV&ITGB5, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. |
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Human ITGAV&ITGB5-on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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Loaded Anti-alpha V Ab., hFc Tag on ProA-Biosensor can bind Biotinylated Human ITGAV&ITGB5, His-Avi Tag with an affinity constant of 57.4 nM as determined in BLI assay . |
Other Available Proteins:
Finding available proteins...
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