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Non-biotinylated Human PDGF R alpha/PDGFRA Protein, Ultra Low Endotoxin (PFR-HM40A-UL) Summary
Product Description |
Recombinant Human PDGF R alpha/PDGFRA is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Glu524. |
Product Background |
Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Non-biotinylated Human PDGF R alpha/PDGFRA Protein, Ultra Low Endotoxin (PFR-HM40A-UL) Specifications/Details
Protein |
PDGF R alpha/PDGFRA |
Synonyms |
PDGFRA; CD140A; PDGFR2; RHEPDGFRA; MGC74795; PDGFR-2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln24-Glu524 |
Molecular Weight |
The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human PDGF R alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human PDGF R alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA. |
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Human PDGF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human PDGF R alpha is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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