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Non-biotinylated Human SIRP gamma/CD172g Protein, Ultra Low Endotoxin (SRP-HM40G-UL) Summary
Product Description |
Recombinant Human SIRP gamma/CD172g Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu29-Pro360. |
Product Background |
Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins, ADC targets |
Non-biotinylated Human SIRP gamma/CD172g Protein, Ultra Low Endotoxin (SRP-HM40G-UL) Specifications/Details
Protein |
SIRP gamma/CD172g |
Synonyms |
CD172g; FLJ42230; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPG; Cripto-1; bA77C3.1; SIRP β 2; SIRPγ; SIRP γ |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu29-Pro360 |
Molecular Weight |
The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human SIRP gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag (Cat. CD7-HM247) with the EC50 of 2.46ug/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human SIRP gamma, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD47, hFc Tag (Cat. CD7-HM247) with the EC50 of 2.46ug/ml determined by ELISA. |
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Human SIRP gamma on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.. |
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The purity of Human SIRP gamma is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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