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Non-biotinylated Mouse TNFSF15 Protein (active trimer), Ultra Low Endotoxin (FSF-MM415-UL) Summary
Product Description |
Recombinant Mouse TNFSF15 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala61-Leu252. |
Product Background |
TL1A and its functional receptor DR3 are members of the TNF/TNFR superfamilies of proteins.TL1A and DR3 are abundantly localized at inflamed intestinal areas of patients with IBD and mice with experimental ileitis or colitis and actively participate in the immunological pathways that underlie mucosal homeostasis and intestinal inflammation.Recently, an important role was demonstrated for TL1A/DR3 as potential mediators of intestinal fibrosis that is associated with the presence of gut inflammation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Non-biotinylated Mouse TNFSF15 Protein (active trimer), Ultra Low Endotoxin (FSF-MM415-UL) Specifications/Details
Protein |
TNFSF15 |
Synonyms |
TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala61-Leu252 |
Molecular Weight |
The protein has a predicted MW of 24.40 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse TNFSF15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.96 nM as determined in SPR assay (Biacore T200). |
Product Tag |
N-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Mouse DR3, His Tag immobilized on CM5 Chip can bind Mouse TNFSF15, His Tag with an affinity constant of 0.96 nM as determined in SPR assay (Biacore T200). |
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Immobilized Mouse TNFSF15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). |
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Immobilized Mouse TNFSF15, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA. |
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Mouse TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse TNFSF15 is greater than 90% and the molecular weight of this protein is around 70-95 kDa as determined by SEC-MALS. |
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The purity of Mouse TNFSF15 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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