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Non-biotinylated SARS-COV-2 Spike S1 Protein, Ultra Low Endotoxin (COV-VM5S1-UL) Summary
Product Description |
Recombinant SARS-COV-2 Spike S1 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Gln14-Arg683. |
Product Background |
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Non-biotinylated SARS-COV-2 Spike S1 Protein, Ultra Low Endotoxin (COV-VM5S1-UL) Specifications/Details
Protein |
Spike S1 |
Synonyms |
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 |
Accession |
|
Species |
SARS-COV-2 |
Biotinylated |
no |
Amino Acid Range |
Gln14-Arg683 |
Molecular Weight |
The protein has a predicted MW of 102.6 kDa. Due to glycosylation, the protein migrates to 130-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 92.3ng/ml determined by ELISA (QC Test). The affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1)-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 92.3ng/ml determined by ELISA (QC Test). |
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SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded SARS-COV-2 Spike S1, hFc Tag on ProA-Biosensor can bind Non-biotinylated Human ACE2, His Tag with an affinity constant of 0.66 nM as determined in BLI assay . |
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The purity of SARS-COV-2 Spike S1 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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