ELISA binding curve showing the dose-dependent interaction between PE-labeled Human HLA-A02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag, and Anti-HLA-A02:01&B2M&AFP Antibody, hFc Tag. The plot presents mean absorbance (OD450) versus the log concentration of the antibody (µg/ml), demonstrating a sigmoidal response.
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Activity: Immobilized PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 36.8ng/ml determined by ELISA.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide
Catalog #MHC-HM407TP
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PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein (MHC-HM407TP) Summary

Product Description

 Recombinant PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide.

Product Background

 Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.

Product Category

 Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-A*02:01&B2M&AFP (FMNKFIYEI) Specifications

Protein
 HLA-A*02:01&B2M&AFP (FMNKFIYEI)
Synonyms
 Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC

MHC Form

 Tetramer

Allele

 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide
Activity
 Immobilized PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 36.8ng/ml determined by ELISA.
Product Tag
 C-His-Avi

Fluorophore

 PE-Labeled
Expression System
 HEK293

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in PBS, 0.2% BSA (pH 7.4).
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 36.8ng/ml determined by ELISA. Immobilized PE-Labeled Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 36.8ng/ml determined by ELISA.
Additional Information
Learn More: MHC Collection Learn More: Peptide-ready MHC Protocol: MHC Staining Case Study: Peptide-ready MHC for TCR Staining Poster: Engineering MHCs as Versatile Reagents Publication: Use of our Peptide-Ready MHCs in CAR-T Cell Research Product Brochure
Other Available Proteins:
Finding available proteins...
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