PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein (M

Express System: E.coli
Product Tag: C-His-Avi
Activity: Immobilized PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide

Catalog #MHC-HE045TP

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$1,050.00

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PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein (MHC-HE045TP) Summary

Product Description	Recombinant PE-Labeled Human HLA-A02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. PE-Labeled Human HLA-A02:01&B2M&PRAME (SLLQHLIGL) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide.
Product Background	PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

Protein	HLA-A*02:01&B2M&PRAME (SLLQHLIGL)
Synonyms	PRAME; OIP-4; OIP4; MAPE;
MHC Form	Tetramer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLQHLIGL
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide
Activity	Immobilized PE-Labeled Human HLA-A02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for HLA-A02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA.
Product Tag	C-His-Avi
Fluorophore	PE-Labeled
Expression System	E.coli

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl, 0.2% BSA (pH 8.0).
Stability And Storage	Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized PE-Labeled Human HLA-A02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for HLA-A02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA.
	1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer (Cat. No.MHC-HE045TP) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal.

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