- Express System: HEK293
- Product Tag: C-His-Avi
- Activity: Immobilized PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 11.0ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide
Product Description |
Recombinant PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLLQHLIGL peptide. |
Product Background |
PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited. |
Product Category |
Recombinant Protein |
Target |
HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer |
MHC Form |
Tetramer |
Allele |
HLA-A*02:01 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide |
Activity |
Immobilized PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 11.0ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Fluorophore |
PE |
Express System |
HEK293 |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS, 0.2% BSA (pH 7.4). |
Stability And Storage |
Valid for 6 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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