PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein (MHC-HM443TP)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Activity: Immobilized PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide
  • Other Available Proteins:
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Catalog #MHC-HM443TP
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PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein (MHC-HM443TP) Summary

Product Description

 Recombinant PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLLQHLIGL peptide.

Product Background

 PRAME (PReferentially expressed Antigen in MElanoma) is a melanoma-associated antigen expressed in cutaneous and ocular melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

Product Category

 Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Specifications

Protein
 HLA-A*02:01&B2M&PRAME (SLLQHLIGL)
Synonyms
 PRAME; OIP-4; OIP4; MAPE;

MHC Form

 Tetramer

Allele

 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQHLIGL peptide
Activity
 Immobilized PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA.
Product Tag
 C-His-Avi

Fluorophore

 PE-Labeled
Expression System
 HEK293

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 0.2% BSA (pH 7.4).
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA. Immobilized PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for HLA-A*02:01&B2M&PRAME TCR, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA.
1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer (Cat. No.MHC-HM443TP) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal. 1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Labeled Human HLA-A*02:01&B2M&PRAME (SLLQHLIGL) Tetramer (Cat. No.MHC-HM443TP) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal.
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