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PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer-Protein (MHC-HM43RTP) Summary
Product Description |
Recombinant PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer is assembled by biotinylated monomer and PE-Labeled streptavidinIt contains Gly25-Thr305 (HLA-A*02:01) and Ile21-Met119 (B2M). |
Product Background |
Peptide Ready HLA-A*02:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-ready MHCs |
Peptide Ready HLA-A*02:01&B2M Specifications
Protein |
Peptide Ready HLA-A*02:01&B2M |
Synonyms |
MHC; HLA-A*02:01; Peptide Ready |
MHC Form |
Tetramer |
Allele |
HLA-A*02:01 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01) & Ile21-Met119(B2M) |
Molecular Weight |
The protein has a predicted MW of 500 kDa. |
Activity |
Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
PE-Labeled |
Expression System |
HEK293 |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS, 100mM L-arginine, 0.2% BSA (pH 7.4). |
Stability And Storage |
Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA. |
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1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein (Cat. MHC-HM43RTP) (Loaded PRAME Peptide SLLQHLIGL) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal. |
Additional Information
Other Available Proteins:
Finding available proteins...
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