• Express System: HEK293
  • Product Tag: C-His-Avi
  • Activity: Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01) & Ile21-Met119(B2M)
Catalog #MHC-HM43RTP
Price
Regular price $1,05000
Regular price Sale price $1,05000
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Size
Avantor VWR Fisher Scientific

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PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer-Protein (MHC-HM43RTP) Summary

Product Description

 Recombinant PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer is assembled by biotinylated monomer and PE-Labeled streptavidinIt contains Gly25-Thr305 (HLA-A*02:01) and Ile21-Met119 (B2M).

Product Background

 Peptide Ready HLA-A*02:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Peptide Ready HLA-A*02:01&B2M Specifications

Protein
 Peptide Ready HLA-A*02:01&B2M
Synonyms
 MHC; HLA-A*02:01; Peptide Ready
MHC Form
 Tetramer
Allele
 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly25-Thr305(HLA-A*02:01) & Ile21-Met119(B2M)
Molecular Weight
 The protein has a predicted MW of 500 kDa.
Activity
 Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 PE-Labeled
Expression System
 HEK293

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 100mM L-arginine, 0.2% BSA (pH 7.4).
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA. Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA.
1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein (Cat. MHC-HM43RTP) (Loaded PRAME Peptide SLLQHLIGL) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal. 1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein (Cat. MHC-HM43RTP) (Loaded PRAME Peptide SLLQHLIGL) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal.
Additional Information
Learn More: MHC Collection Learn More: Peptide-ready MHC Protocol: MHC Staining Case Study: Peptide-ready MHC for TCR Staining Poster: Engineering MHCs as Versatile Reagents Publication: Use of our Peptide-Ready MHCs in CAR-T Cell Research Product Brochure
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