Name: PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer-Protein (MHC-HM43RTP)
Brand: KactusBio
SKU: MHC-HM43RTP-100UG
Price: 1050.0 USD
Availability: InStock

PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer-Protein (MHC-HM43RTP)

Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA.

1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein (Cat. MHC-HM43RTP) (Loaded PRAME Peptide SLLQHLIGL) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal.

Express System: HEK293
Product Tag: C-His-Avi
Activity: Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly25-Thr305 (HLA-A*02:01) & Ile21-Met119 (B2M)

Catalog #MHC-HM43RTP

Size

100UG

500UG

Order by 4pm ET for delivery on:

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PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer-Protein (MHC-HM43RTP) Summary

Product Description	Recombinant PE-Labeled Human Peptide Ready HLA-A02:01&B2M Tetramer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human Peptide Ready HLA-A02:01&B2M Tetramer is assembled by biotinylated monomer and PE-Labeled streptavidinIt contains Gly25-Thr305 (HLA-A*02:01) and Ile21-Met119 (B2M).
Product Background	Peptide Ready HLA-A02:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-ready MHCs

Peptide Ready HLA-A*02:01&B2M Specifications

Protein	Peptide Ready HLA-A*02:01&B2M
Synonyms	MHC; HLA-A*02:01; Peptide Ready
MHC Form	Tetramer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305 (HLA-A*02:01) & Ile21-Met119 (B2M)
Activity	Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Fluorophore	PE
Expression System	HEK293
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 100mM L-arginine, 0.2% BSA (pH 7.4).
Stability And Storage	Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4 ng/ml determined by ELISA.
	1E6 of PRAME specific TCR-HEK293T cell line were stained with 100ul of 10ug/ml PE-Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein (Cat. MHC-HM43RTP) (Loaded PRAME Peptide SLLQHLIGL) and non-transfected HEK293T cells and PE-Labeled protein were used as negative control. The binding activity was evaluated by PE signal.