PE-Labeled Human Peptide Ready HLA-A*11:01&B2M Tetramer-Protein (MHC-HM41RTP)
Catalog: MHC-HM41RTP
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Product Description | Recombinant PE-Labeled Human Peptide Ready HLA-A*11:01&B2M Tetramer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human Peptide Ready HLA-A*11:01&B2M Tetramer is assembled by biotinylated monomer and PE-Labeled streptavidin.It contains Gly25-Thr305 (HLA-A*11:01) and Ile21-Met119 (B2M). |
Product Background |
Peptide Ready HLA-A*11:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*11:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. |
Product Category |
Recombinant Protein |
Target |
Peptide Ready HLA-A*11:01&B2M Tetramer |
MHC Form | Tetramer |
Allele | HLA-A*11:01 |
Gene/Protein ID |
AAV53343.1(HLA-A*11:01)&P61769(B2M) |
Species |
Human |
Exact Sequence |
Gly25-Thr305(HLA-A*11:01) & Ile21-Met119(B2M) |
Activity |
Immobilized PE-Labeled Human Peptide Ready HLA-A*11:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Fluorophore |
PE |
Express System |
HEK293 |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22 um filtered solution in PBS, 0.2% BSA (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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