PE-Labeled Human Peptide Ready HLA-A*11:01&B2M Tetramer-Protein (MHC-HM41RTP)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Activity: Immobilized PE-Labeled Human Peptide Ready HLA-A*11:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*11:01) & Ile21-Met119(B2M)

Catalog: MHC-HM41RTP

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Product Description Recombinant PE-Labeled Human Peptide Ready HLA-A*11:01&B2M Tetramer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Human Peptide Ready HLA-A*11:01&B2M Tetramer is assembled by biotinylated monomer and PE-Labeled streptavidin.It contains Gly25-Thr305 (HLA-A*11:01) and Ile21-Met119 (B2M).

Product Background

 Peptide Ready HLA-A*11:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*11:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Product Category

 Recombinant Protein

Target

 Peptide Ready HLA-A*11:01&B2M Tetramer
MHC Form Tetramer
Allele HLA-A*11:01

Gene/Protein ID

 AAV53343.1(HLA-A*11:01)&P61769(B2M)

Species

 Human

Exact Sequence

 Gly25-Thr305(HLA-A*11:01) & Ile21-Met119(B2M)

Activity

 Immobilized PE-Labeled Human Peptide Ready HLA-A*11:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His-Avi

Fluorophore

 PE

Express System

 HEK293
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS, 0.2% BSA (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized PE-Labeled Human Peptide Ready HLA-A*11:01&B2M Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.

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