• Express System: HEK293
  • Product Tag: C-His-Avi
  • Amino Acid Range: His24-Pro297(H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide
Catalog #MHC-MM453TP
Price
Regular price $1,05000
Regular price Sale price $1,05000
/
Size
Avantor VWR Fisher Scientific

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PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer Protein (MHC-MM453TP) Summary

Product Description

 Recombinant PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin.It contains His24-Pro297 (H-2K(b)), Ile21-Met119 (B2M) and SIINFEKL peptide.

Product Background

 Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature.

H-2K(b)&B2M&OVA (SIINFEKL) Specifications

Protein
 H-2K(b)&B2M&OVA (SIINFEKL)
Synonyms
 H-2K; OVA; MHC; H2-K1;;H2K; H2K1
MHC Form
 Tetramer
Allele
 H-2K(b)
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 His24-Pro297(H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide
Product Tag
 C-His-Avi
Fluorophore
 PE-Labeled
Expression System
 HEK293

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 0.2% BSA (pH 7.4).
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer can specifically bind to CD8+, OVA-specific T cells from OT-I TCR transgenic mice. Cells were incubated with 10 ug/ml PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer. PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer can specifically bind to CD8+, OVA-specific T cells from OT-I TCR transgenic mice. Cells were incubated with 10 ug/ml PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer.
Additional Information
Learn More: MHC Collection Learn More: Peptide-ready MHC Protocol: MHC Staining Case Study: Peptide-ready MHC for TCR Staining Poster: Engineering MHCs as Versatile Reagents Publication: Use of our Peptide-Ready MHCs in CAR-T Cell Research Product Brochure
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