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PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer Protein (MHC-MM453TP) Summary
Product Description |
Recombinant PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin.It contains His24-Pro297 (H-2K(b)), Ile21-Met119 (B2M) and SIINFEKL peptide. |
Product Background |
Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
H-2K(b)&B2M&OVA (SIINFEKL) Specifications
Protein |
H-2K(b)&B2M&OVA (SIINFEKL) |
Synonyms |
H-2K; OVA; MHC; H2-K1; |
MHC Form |
Tetramer |
Accession |
P01901(H-2K(b))&P01887(B2M)&SIINFEKL |
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
His24-Pro297(H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide |
Product Tag |
C-His-Avi |
Fluorophore |
PE-Labeled |
Expression System |
HEK293 |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS, 0.2% BSA (pH 7.4). |
Stability And Storage |
Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer can specifically bind to CD8+, OVA-specific T cells from OT-I TCR transgenic mice. Cells were incubated with 10 ug/ml PE-Labeled Mouse H-2K(b)&B2M&OVA (SIINFEKL) Tetramer. |
Additional Information
Other Available Proteins:
Finding available proteins...
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