Product Description |
Recombinant Rabbit IgG Protein is expressed from HEK293 without tag.It contains Ser101-Lys323 (T185A, N284S). |
Product Background |
Immunoglobulin G (IgG) glycosylation can modulate antibody effector functions. Depending on the precise composition of the sugar moiety attached to individual IgG glycovariants either pro- or anti-inflammatory effector pathways can be initiated via differential binding to type I or type II Fc-receptors. Rabbits make high-affinity IgG antibodies, all of which bind with high affinity to Protein A from Staphylococcus aureus and Protein G from Group G Streptococcus. |
Product Category |
Recombinant Protein |
Target |
IgG |
Accession |
|
Species |
Rabbit |
Biotinylated |
no |
Amino Acid Range |
Ser101-Lys323(T185A, N284S) |
Molecular Weight |
The protein has a predicted MW of 25.13 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Rabbit IgG, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 10.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
No Tag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS, 150mM Nacl, 10% Glycerol (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized Rabbit IgG, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 10.5ng/ml determined by ELISA. |
|
|
Rabbit IgG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Rabbit IgG is greater than 95% as determined by SEC-HPLC. |
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