Rabbit IgG Protein (IGG-RM001)

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Rabbit IgG, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 10.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser101-Lys323(T185A, N284S)

Catalog: IGG-RM001

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Product Description	Recombinant Rabbit IgG Protein is expressed from HEK293 without tag.It contains Ser101-Lys323 (T185A, N284S).
Product Background	Immunoglobulin G (IgG) glycosylation can modulate antibody effector functions. Depending on the precise composition of the sugar moiety attached to individual IgG glycovariants either pro- or anti-inflammatory effector pathways can be initiated via differential binding to type I or type II Fc-receptors. Rabbits make high-affinity IgG antibodies, all of which bind with high affinity to Protein A from Staphylococcus aureus and Protein G from Group G Streptococcus.
Product Category	Recombinant Protein

Target	IgG
Gene/Protein ID	P01870
Species	Rabbit
Exact Sequence	Ser101-Lys323(T185A, N284S)
Molecular Weight	The protein has a predicted MW of 25.13 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Rabbit IgG, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 10.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 150mM Nacl, 10% Glycerol (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Rabbit IgG, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 10.5ng/ml determined by ELISA.
	The purity of Rabbit IgG is greater than 95% as determined by SEC-HPLC.
	Rabbit IgG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.