ELISA binding curve showing dose-dependent interaction between rabbit IgG and biotinylated human Fc gamma RI (His tag), with OD450 measurement.
Rabbit IgG Protein (IGG-RM001)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rabbit IgG, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ser101-Lys323(T185A, N284S)
Catalog #IGG-RM001
Regular price $83.00
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Size
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Rabbit IgG Protein (IGG-RM001) Summary

Product Description

Recombinant Rabbit IgG Protein is expressed from HEK293 without tag.It contains Ser101-Lys323 (T185A, N284S).

Product Background

Immunoglobulin G (IgG) glycosylation can modulate antibody effector functions. Depending on the precise composition of the sugar moiety attached to individual IgG glycovariants either pro- or anti-inflammatory effector pathways can be initiated via differential binding to type I or type II Fc-receptors. Rabbits make high-affinity IgG antibodies, all of which bind with high affinity to Protein A from Staphylococcus aureus and Protein G from Group G Streptococcus.

Product Category

Recombinant Protein / Antibody discovery / Fc receptors

Rabbit IgG Protein (IGG-RM001) Specifications/Details

Protein
IgG
Synonyms
IgG;
Accession
Species
Rabbit
Biotinylated
no
Amino Acid Range
Ser101-Lys323(T185A, N284S)
Molecular Weight
The protein has a predicted MW of 25.13 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Rabbit IgG, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).
Product Tag
No Tag

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS, 150mM NaCl, 10% Glycerol (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Rabbit IgG, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test). Immobilized Rabbit IgG, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RI, His Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).
Rabbit IgG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rabbit IgG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rabbit IgG is greater than 95% as determined by SEC-HPLC. The purity of Rabbit IgG is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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