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Rat ANGPT2/Angiopoietin-2 Protein, Ultra Low Endotoxin (APN-RM102-UL) Summary
Product Description |
Recombinant Rat ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Lys275-Phe496. |
Product Background |
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Rat ANGPT2/Angiopoietin-2 Protein, Ultra Low Endotoxin (APN-RM102-UL) Specifications/Details
Protein |
ANGPT2/Angiopoietin-2 |
Synonyms |
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand |
Accession |
|
Species |
Rat |
Biotinylated |
no |
Amino Acid Range |
Lys275-Phe496 |
Molecular Weight |
The protein has a predicted MW of 26.28 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Rat ANGPT2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Rat ANGPT2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. |
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Rat ANGPT2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Rat ANGPT2 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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