• Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Rat APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag (Cat. BCM-MM217) with the EC50 of 0.6ng/ml determined by ELISA.
  • Amino Acid Range: Ala96-Leu241
Catalog #APR-RM113
Price
Regular price $34500
Regular price Sale price $34500
/
Size
Avantor VWR Fisher Scientific

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Rat APRIL/TNFSF13 Protein (APR-RM113) Summary

Product Description

 Recombinant Rat APRIL/TNFSF13 Protein is expressed from HEK293 with His tag and Flag tag at the N-terminus.It contains Ala96-Leu241.

Product Background

 The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.

Rat APRIL/TNFSF13 Protein (APR-RM113) Specifications/Details

Protein
 APRIL/TNFSF13
Synonyms
 CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2
Accession
Species
 Rat
Biotinylated
 no
Amino Acid Range
 Ala96-Leu241
Molecular Weight
 The protein has a predicted MW of 18.20 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Rat APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag (Cat. BCM-MM217) with the EC50 of 0.6ng/ml determined by ELISA.
Product Tag
 N-His-Flag
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Rat APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag (Cat. BCM-MM217) with the EC50 of 0.6ng/ml determined by ELISA. Immobilized Rat APRIL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag (Cat. BCM-MM217) with the EC50 of 0.6ng/ml determined by ELISA.
Rat APRIL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rat APRIL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat APRIL is greater than 90% as determined by SEC-HPLC. The purity of Rat APRIL is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...