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Rat B7-H2/ICOSLG Protein, Ultra Low Endotoxin (BH7-RM172-UL) Summary
Product Description |
Recombinant Rat B7-H2/ICOSLG Protein is expressed from HEK293 with His tag at the C-terminus.It contains Glu25-Lys261. |
Product Background |
B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Rat B7-H2/ICOSLG Protein, Ultra Low Endotoxin (BH7-RM172-UL) Specifications/Details
Protein |
B7-H2/ICOSLG |
Synonyms |
CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50 |
Accession |
|
Species |
Rat |
Biotinylated |
no |
Amino Acid Range |
Glu25-Lys261 |
Molecular Weight |
The protein has a predicted MW of 28.52 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Rat B7-H2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 28.2ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Rat B7-H2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse ICOS, hFc Tag with the EC50 of 28.2ng/ml determined by ELISA. |
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Rat B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Mouse ICOS, hFc Tag on ProA-Biosensor can bind Rat B7-H2, His Tag with an affinity constant of 0.40 µM as determined in BLI assay . |
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The purity of Rat B7-H2 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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