Rat CD28 Protein (CD8-RM128)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: The affinity constant of 42.64 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asn20-Leu150

Catalog: CD8-RM128

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Product Description Recombinant Rat CD28 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Asn20-Leu150.

Product Background

CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Product Category

Recombinant Protein

Target

CD28
Accession

Species

Rat

Amino Acid Range

Asn20-Leu150
Molecular Weight The protein has a predicted MW of 16.66 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 42.64 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Rat CD28, His Tag with an affinity constant of 42.64 uM as determined in SPR assay (Biacore T200).
Rat CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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