Rat CD28 Protein (CD8-RM128)
Rat CD28 Protein (CD8-RM128)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: The affinity constant of 42.64 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asn20-Leu150
Catalog #CD8-RM128
Regular price $270.00
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Product Description

Recombinant Rat CD28 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Asn20-Leu150.

Product Background

CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

CD28

Synonyms

CD28 antigen (Tp44); CD28 molecule; CD28

Accession

Species

Rat

Biotinylated

no

Amino Acid Range

Asn20-Leu150

Molecular Weight

The protein has a predicted MW of 16.66 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.

Activity

The affinity constant of 42.64 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Rat CD28, His Tag with an affinity constant of 42.64 uM as determined in SPR assay (Biacore T200). Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Rat CD28, His Tag with an affinity constant of 42.64 uM as determined in SPR assay (Biacore T200).
Rat CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rat CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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