Rat CD36/SR-B3 Protein (CD3-RM136)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rat CD36, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD36 Antibody, Rabbit IgG Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly30-Lys439
Catalog #CD3-RM136
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Product Description
 Recombinant Rat CD36/SR-B3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly30-Lys439.
Product Background
 CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions.CD36 is a multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides.
Product Category
 Recombinant Protein
Target
 CD36/SR-B3
Accession
Species
 Rat
Biotinylated
 no
Amino Acid Range
 Gly30-Lys439
Molecular Weight
 The protein has a predicted MW of 47.46 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Rat CD36, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD36 Antibody, Rabbit IgG Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Rat CD36, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD36 Antibody, Rabbit IgG Tag with the EC50 of 7.9ng/ml determined by ELISA.
Rat CD36 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat CD36 is greater than 95% as determined by SEC-HPLC.
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