Rat CD36/SR-B3 Protein (CD3-RM136)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rat CD36, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD36 Antibody, Rabbit IgG Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly30-Lys439

Catalog: CD3-RM136

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Product Description Recombinant Rat CD36/SR-B3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly30-Lys439.

Product Background

CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions.CD36 is a multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides.

Product Category

Recombinant Protein

Target

CD36/SR-B3
Accession

Species

Rat

Amino Acid Range

Gly30-Lys439
Molecular Weight The protein has a predicted MW of 47.46 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Rat CD36, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD36 Antibody, Rabbit IgG Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Rat CD36, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD36 Antibody, Rabbit IgG Tag with the EC50 of 7.9ng/ml determined by ELISA.
The purity of Rat CD36 is greater than 95% as determined by SEC-HPLC.
Rat CD36 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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