Product Description |
Recombinant Rat CD36/SR-B3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly30-Lys439. |
Product Background |
CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions.CD36 is a multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
CD36/SR-B3 |
Synonyms |
CD36; FAT; FATCHDS7; GPIIIb; GPIV; PAS IV; PAS-4; SCARB3; SRB3; SR-B3; BDPLT10; CHDS7; GP3B |
Accession |
|
Species |
Rat |
Biotinylated |
no |
Amino Acid Range |
Gly30-Lys439 |
Molecular Weight |
The protein has a predicted MW of 47.46 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Rat CD36, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD36 Antibody, Rabbit IgG Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Rat CD36, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD36 Antibody, Rabbit IgG Tag with the EC50 of 7.9ng/ml determined by ELISA. |
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Rat CD36 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Rat CD36 is greater than 95% as determined by SEC-HPLC. |
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