Rat Coagulation factor II-Protein (CFR-RM102)
Rat Coagulation factor II-Protein (CFR-RM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gln25-Arg617
Catalog #CFR-RM102
Regular price $315.00
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Product Description

Recombinant Rat Coagulation factor II-Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-Arg617.

Product Background

Prothrombin, or coagulation factor II, is a multidomain zymogen precursor of thrombin that undergoes an allosteric equilibrium between two alternative conformations, open and closed, that react differently with the physiological activator prothrombinase. It has 10 sites of gamma-carboxylation, which are required for its bioactivity, and is N-glycosylated at three of four putative sites.

Product Category

Recombinant Protein / Antibody discovery

Protein

Coagulation factor II

Synonyms

F2; Prothrombin; thrombin; F II; FII

Accession

Species

Rat

Biotinylated

no

Amino Acid Range

Gln25-Arg617

Molecular Weight

The protein has a predicted MW of 69.12 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Rat Coagulation factor II on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rat Coagulation factor II on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat Coagulation factor II is greater than 95% as determined by SEC-HPLC. The purity of Rat Coagulation factor II is greater than 95% as determined by SEC-HPLC.

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