Rat CTLA-4/CD152 Protein (CTL-RM141)

Immobilized Rat CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, hFc Tag (Cat. B71-HM280) with the EC50 of 1.3ng/ml determined by ELISA.

Rat CTLA-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Rat CTLA-4 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Rat CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, hFc Tag (Cat. B71-HM280) with the EC50 of 1.3ng/ml determined by ELISA.
Amino Acid Range: Ile38-Asp161

Catalog #CTL-RM141

Price

$345⁰⁰

Size

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Rat CTLA-4/CD152 Protein (CTL-RM141) Summary

Product Description

Recombinant Rat CTLA-4/CD152 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ile38-Asp161.

Product Background

CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.

Rat CTLA-4/CD152 Protein (CTL-RM141) Specifications/Details

Protein	CTLA-4/CD152
Synonyms	CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12
Accession	NP_113862.1
Species	Rat
Biotinylated	no
Amino Acid Range	Ile38-Asp161
Molecular Weight	The protein has a predicted MW of 15.16 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Rat CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, hFc Tag (Cat. B71-HM280) with the EC50 of 1.3ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Rat CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, hFc Tag (Cat. B71-HM280) with the EC50 of 1.3ng/ml determined by ELISA.
	Rat CTLA-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Rat CTLA-4 is greater than 95% as determined by SEC-HPLC.