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Rat Fc gamma RI/CD64 Protein (FRI-RM164) Summary
Product Description |
Recombinant Rat Fc gamma RI/CD64 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln16-Pro285. |
Product Background |
FcgammaRIV is a relatively new IgG Fc receptor (FcgammaR) that is reported to contribute to the pathogenesis of autoimmune diseases.FcgammaRIII and FcgammaRIV are each essential to trigger an FcRgamma-linker for activation of T-cell-dependent signal that drives C5a production in the Arthus reaction. A combined requirement for FcgammaRIII and FcgammaRIV in autoimmune injury, and identify the linker for activation of T cells adaptor as an integral component of linked FcgammaR and C5a anaphylatoxin receptor activation to generate inflammation. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Rat Fc gamma RI/CD64 Protein (FRI-RM164) Specifications/Details
Protein |
Fc gamma RIV/CD16-2 |
Synonyms |
CD16-2; FcgammaRIV; Fcgr4; FcγRIV |
Accession |
|
Species |
Rat |
Biotinylated |
no |
Amino Acid Range |
Gln16-Pro285 |
Molecular Weight |
The protein has a predicted MW of 31.86 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.74 µM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Rat Fc gamma RI, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.74 µM as determined in SPR assay (Biacore T200). |
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Rat Fc gamma RI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Rat Fc gamma RI, His Tag on Anti-His-Biosensor can bind Trastuzumab with an affinity constant of 0.11 µM as determined in BLI assay . |
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The purity of Rat Fc gamma RI is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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