Regular price
$264.00
Order by 4pm ET for delivery on:
Rat Fc gamma RI/CD64 Protein, Ultra Low Endotoxin (FRI-RM164-UL) Summary
Product Description |
Recombinant Rat Fc gamma RI/CD64 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln16-Pro285. |
Product Background |
FcgammaRIV is a relatively new IgG Fc receptor (FcgammaR) that is reported to contribute to the pathogenesis of autoimmune diseases.FcgammaRIII and FcgammaRIV are each essential to trigger an FcRgamma-linker for activation of T-cell-dependent signal that drives C5a production in the Arthus reaction. A combined requirement for FcgammaRIII and FcgammaRIV in autoimmune injury, and identify the linker for activation of T cells adaptor as an integral component of linked FcgammaR and C5a anaphylatoxin receptor activation to generate inflammation. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Rat Fc gamma RI/CD64 Protein, Ultra Low Endotoxin (FRI-RM164-UL) Specifications/Details
Protein |
Fc gamma RIV/CD16-2 |
Synonyms |
CD16-2; FcgammaRIV; Fcgr4; FcγRIV |
Accession |
|
Species |
Rat |
Biotinylated |
no |
Amino Acid Range |
Gln16-Pro285 |
Molecular Weight |
The protein has a predicted MW of 31.86 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.74 µM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Rat Fc gamma RI, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.74 µM as determined in SPR assay (Biacore T200). |
|
|
Rat Fc gamma RI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Loaded Rat Fc gamma RI, His Tag on Anti-His-Biosensor can bind Trastuzumab with an affinity constant of 0.11 µM as determined in BLI assay . |
|
|
The purity of Rat Fc gamma RI is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
