• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 3.26 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ala23-Ser298(FCGRT) & Ile21-Met119(B2M)
Catalog #FRN-RM101
Price
Regular price $35530
Regular price Sale price $35530
/
Size
Avantor VWR Fisher Scientific

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Rat FcRn Protein (FRN-RM101) Summary

Product Description

 Recombinant Rat FcRn Protein is expressed from HEK293 with His tag at the C-terminus of FCGRT.It contains Ala23-Ser298 (FCGRT) and Ile21-Met119 (B2M).

Product Background

 The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.

Rat FcRn Protein (FRN-RM101) Specifications/Details

Protein
 FcRn
Synonyms
 FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
Accession
Species
 Rat
Biotinylated
 no
Amino Acid Range
 Ala23-Ser298(FCGRT) & Ile21-Met119(B2M)
Molecular Weight
 The protein has a predicted MW of 32.60 kDa (FCGRT) and 11.63 kDa (B2M). Due to glycosylation, the protein migrates to 45-60 kDa (FCGRT) and 13-15 kDa (B2M) based on Bis-Tris PAGE result.
Activity
 The affinity constant of 3.26 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Rat FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 3.26 nM as determined in SPR assay (Biacore T200). Rat FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 3.26 nM as determined in SPR assay (Biacore T200).
Rat FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rat FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat FcRn is greater than 95% as determined by SEC-HPLC. The purity of Rat FcRn is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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