Rat FGFR2 beta (IIIb) Protein, Ultra Low Endotoxin (FGF-RM1BB-UL)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Rat FGFR2 beta (IIIb), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
Amino Acid Range: Pro84-Glu308

Catalog #FGF-RM1BB-UL

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$379.50

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Rat FGFR2 beta (IIIb) Protein, Ultra Low Endotoxin (FGF-RM1BB-UL) Summary

Product Description	Recombinant Rat FGFR2 beta (IIIb) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro84-Glu308.
Product Background	Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha- isoform, or only IgII and IgIII, referred to as the beta- isoform.
Product Category	Recombinant Protein / Antibody discovery / ADC targets

Protein	FGFR2 beta (IIIb)
Synonyms	Fibroblast growth factor receptor 2;FGFR-2;KGFR;K-sam;Keratinocyte growth factor receptor;CD332;BEK;KSAM
Accession	F1LRU8
Species	Rat
Biotinylated	no
Amino Acid Range	Pro84-Glu308
Molecular Weight	The protein has a predicted MW of 26.86 kDa. Due to glycosylation, the protein migrates to 60-90 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Rat FGFR2 beta (IIIb), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Rat FGFR2 beta (IIIb), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
	Rat FGFR2 beta (IIIb) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-FGFR2 (IIIb) Antibody, hFc Tag on ProA-Biosensor can bind Rat FGFR2 beta (IIIb), His Tag with an affinity constant of 5.10 nM as determined in BLI assay.
	The purity of Rat FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC.

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