Rat GARP&Latent TGF Beta 1 Complex Protein (GAT-RM401)
Catalog: GAT-RM401
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| Product Description | Recombinant Rat GARP&Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi Tag at the C-Terminus.It contains Ile18-Asn628(GARP)&Leu30-Arg278(Latent TGF Beta 1). |
| Product Category | Recombinant Protein |
| Target | GARP&Latent TGF Beta 1 Complex |
| Gene/Protein ID | D3ZVD5(GARP)&P17246(Latent TGF Beta) |
| Species | Rat |
| Exact Sequence | Ile18-Asn628(GARP) & Leu30-Arg278(Latent TGF Beta 1) |
| Molecular Weight | The protein has a predicted MW of 69.80 kDa (GARP)&41.28 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&42-48 kDa&13 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result. |
| Activity | Immobilized Rat GARP&Latent TGF Beta 1 Complex, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 2.0ug/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Rat GARP&Latent TGF Beta 1 Complex, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 2.0ug/ml determined by ELISA. |
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The purity of Rat GARP&Latent TGF Beta 1 Complex is greater than 95% as determined by SEC-HPLC. |
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Rat GARP&Latent TGF Beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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