Rat GDF15 Protein (GDF-RE115)
Catalog: GDF-RE115
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Product Description | Recombinant Rat GDF15 Protein is expressed from E.coli with His tag at the N-terminal.It contains Ser189-Ala303. |
Product Category | Recombinant Protein |
Target | GDF15 |
Gene/Protein ID | Q9Z0J6 |
Species | Rat |
Exact Sequence | Ser189-Ala303 |
Molecular Weight | The protein has a predicted MW of 13.68 kDa. The protein migrates to 15-17 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Rat GDF15, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 6.2μg/ml determined by ELISA. See testing image for detail. |
Product Tag | N-His |
Express System | E.coli |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9). |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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