Name: Rat GDF15 Protein (GDF-RE115)
Brand: KactusBio
SKU: GDF-RE115-100UG
Price: 315.0 USD
Availability: InStock

Express System: E.coli
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Rat GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ser189-Ala303

Catalog #GDF-RE115

Size

100UG

500UG

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Product Description	Recombinant Rat GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ser189-Ala303.
Product Background	Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
Product Category	Recombinant Protein

Protein	GDF15
Synonyms	GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β
Accession	Q9Z0J6
Species	Rat
Biotinylated	no
Amino Acid Range	Ser189-Ala303
Molecular Weight	The protein has a predicted MW of 13.68 kDa. The protein migrates to 15-17 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Rat GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95ug/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Rat GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95ug/ml determined by ELISA.
	Immobilized Rat GDF-15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
	Rat GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.