Rat GDF15 Protein (GDF-RE115)

  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Rat GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser189-Ala303

Catalog: GDF-RE115

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Product Description Recombinant Rat GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ser189-Ala303.

Product Background

Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.

Product Category

Recombinant Protein

Target

GDF15

Gene/Protein ID

Q9Z0J6

Species

Rat

Exact Sequence

Ser189-Ala303
Molecular Weight The protein has a predicted MW of 13.68 kDa. The protein migrates to 15-17 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Rat GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95ug/ml determined by ELISA. See testing image for detail.

Product Tag

N-His

Express System

E.coli
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Rat GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95ug/ml determined by ELISA.
Immobilized Rat GDF-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA (QC Test).
Rat GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

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