Rat GDF15 Protein (GDF-RE115)
Catalog: GDF-RE115
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| Product Description | Recombinant Rat GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ser189-Ala303. |
| Product Category | Recombinant Protein |
| Target | GDF15 |
| Gene/Protein ID | Q9Z0J6 |
| Species | Rat |
| Exact Sequence | Ser189-Ala303 |
| Molecular Weight | The protein has a predicted MW of 13.68 kDa. The protein migrates to 15-17 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Rat GDF-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA (QC Test). See testing image for detail. |
| Product Tag | N-His |
| Express System | E.coli |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9). |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Rat GDF-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA (QC Test). |
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Immobilized Rat GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95ug/ml determined by ELISA. |
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Rat GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%. |
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