Rat GDF15 Protein (GDF-RE115)
Rat GDF15 Protein (GDF-RE115)
Rat GDF15 Protein (GDF-RE115)
  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Rat GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser189-Ala303
Catalog #GDF-RE115
Regular price $450.00
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Product Description
Recombinant Rat GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ser189-Ala303.
Product Background
Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
Product Category
Recombinant Protein
Target
GDF15
Synonyms
GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β
Accession
Species
Rat
Biotinylated
no
Amino Acid Range
Ser189-Ala303
Molecular Weight
The protein has a predicted MW of 13.68 kDa. The protein migrates to 15-17 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Rat GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95ug/ml determined by ELISA. See testing image for detail.
Product Tag
N-His
Express System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Rat GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95ug/ml determined by ELISA.
Immobilized Rat GDF-15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
Rat GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

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