Name: Rat GFRAL/GFR alpha-like Protein (GFL-RM101)
Brand: KactusBio
SKU: GFL-RM101-100UG
Price: 315.0 USD
Availability: InStock

Rat GFRAL/GFR alpha-like Protein (GFL-RM101)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Rat GFRAL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln20-Gly350

Catalog #GFL-RM101

Size

100UG

500UG

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Product Description	Recombinant Rat GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag at the N-terminus.It contains Gln20-Gly350.
Product Background	GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Product Category	Recombinant Protein

Target	GFRAL/GFR alpha-like
Synonyms	GFR alpha-like; GFRAL; GRAL; C6orf144
Accession	D3ZB94
Species	Rat
Biotinylated	no
Amino Acid Range	Gln20-Gly350
Molecular Weight	The protein has a predicted MW of 38.17 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Rat GFRAL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Rat GFRAL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human GDF15, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
	Rat GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Rat GFRAL is greater than 95% as determined by SEC-HPLC.

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