Rat IL-13Ra2 Protein (ILR-RM1R2)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rat IL-13Ra2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu24-Lys336
Catalog #ILR-RM1R2
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Product Description
 Recombinant Rat IL-13Ra2 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu24-Lys336.
Product Background
 Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
Product Category
 Recombinant Protein
Target
 IL-13Ra2
Accession
Species
 Rat
Biotinylated
 no
Amino Acid Range
 Leu24-Lys336
Molecular Weight
 The protein has a predicted MW of 37.51 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Rat IL-13Ra2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Rat IL-13Ra2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.
Rat IL-13Ra2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat IL-13Ra2 is greater than 95% as determined by SEC-HPLC.

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