Rat IL-13Ra2 Protein (ILR-RM1R2)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rat IL-13Ra2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu24-Lys336

Catalog: ILR-RM1R2

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Product Description Recombinant Rat IL-13Ra2 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu24-Lys336.

Product Background

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

Product Category

Recombinant Protein

Target

IL-13Ra2

Gene/Protein ID

Q8VHK6

Species

Rat

Exact Sequence

Leu24-Lys336
Molecular Weight The protein has a predicted MW of 37.51 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Rat IL-13Ra2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Rat IL-13Ra2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.
The purity of Rat IL-13Ra2 is greater than 95% as determined by SEC-HPLC.
Rat IL-13Ra2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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