|Recombinant Rat IL-13Ra2 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu24-Lys336.
|Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
|The protein has a predicted MW of 37.51 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
|Immobilized Rat IL-13Ra2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.