Name: Rat IL-13Ra2 Protein (ILR-RM2R2)
Brand: KactusBio
SKU: ILR-RM2R2-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-13, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA (QC Test). The affinity constant of 93.96 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Leu24-Lys336

Catalog #ILR-RM2R2

Size

100UG

500UG

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Rat IL-13Ra2 Protein (ILR-RM2R2) Summary

Product Description	Recombinant Rat IL-13Ra2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu24-Lys336.
Product Background	Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Rat IL-13Ra2 Protein (ILR-RM2R2) Specifications/Details

Protein	IL-13Ra2
Synonyms	CD213a2; IL-13 R alpha 2; IL13BP; IL13R; IL13RA2; IL-13R subunit alpha-2; IL-13Ra2
Accession	Q8VHK6
Species	Rat
Biotinylated	no
Amino Acid Range	Leu24-Lys336
Molecular Weight	The protein has a predicted MW of 63.17 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-13, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA (QC Test). The affinity constant of 93.96 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Rat IL-13Ra2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13, His Tag with an affinity constant of 93.96 pM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-13, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA (QC Test).
	Rat IL-13Ra2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Rat IL-13Ra2, hFc Tag on ProA-Biosensor can bind Human IL-13, His Tag with an affinity constant of 2.53 nM as determined in BLI assay (Gator® Prime).
	The purity of Rat IL-13Ra2 is greater than 95% as determined by SEC-HPLC.