Rat IL-13Ra2 Protein (ILR-RM2R2)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-13, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc Tag with the EC50 of 10.7ng/ml determined by ELISA (QC Test). The affinity constant of 93.96 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Leu24-Lys336

Catalog: ILR-RM2R2

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Product Description	Recombinant Rat IL-13Ra2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu24-Lys336.
Product Background	Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
Product Category	Recombinant Protein

Target	IL-13Ra2
Gene/Protein ID	Q8VHK6
Species	Rat
Exact Sequence	Leu24-Lys336
Molecular Weight	The protein has a predicted MW of 63.17 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-13, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc Tag with the EC50 of 10.7ng/ml determined by ELISA (QC Test). The affinity constant of 93.96 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Rat IL-13Ra2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13, His Tag with an affinity constant of 93.96 pM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-13, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc Tag with the EC50 of 10.7ng/ml determined by ELISA (QC Test).
	The purity of Rat IL-13Ra2 is greater than 95% as determined by SEC-HPLC.
	Rat IL-13Ra2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.