Product Description |
Recombinant Rat IL-13Ra2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu24-Lys336. |
Product Background |
Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
IL-13Ra2 |
Synonyms |
CD213a2; IL-13 R alpha 2; IL13BP; IL13R; IL13RA2; IL-13R subunit alpha-2; IL-13Ra2 |
Accession |
|
Species |
Rat |
Biotinylated |
no |
Amino Acid Range |
Leu24-Lys336 |
Molecular Weight |
The protein has a predicted MW of 63.17 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-13, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA (QC Test). The affinity constant of 93.96 pM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Rat IL-13Ra2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13, His Tag with an affinity constant of 93.96 pM as determined in SPR assay (Biacore T200). |
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Immobilized Human IL-13, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA (QC Test). |
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Rat IL-13Ra2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Rat IL-13Ra2 is greater than 95% as determined by SEC-HPLC. |
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