Product Description |
Recombinant Rat IL-13Ra2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu24-Lys336. |
Product Background |
Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
IL-13Ra2 |
Synonyms |
CD213a2; IL-13 R alpha 2; IL13BP; IL13R; IL13RA2; IL-13R subunit alpha-2; IL-13Ra2 |
Accession |
|
Species |
Rat |
Biotinylated |
no |
Amino Acid Range |
Leu24-Lys336 |
Molecular Weight |
The protein has a predicted MW of 63.17 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-13, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA (QC Test). The affinity constant of 93.96 pM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Rat IL-13Ra2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13, His Tag with an affinity constant of 93.96 pM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human IL-13, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA (QC Test). |
|
|
Rat IL-13Ra2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Rat IL-13Ra2 is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed
