Rat IL-13Ra2 Protein (ILR-RM2R2)
Catalog: ILR-RM2R2
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Product Description | Recombinant Rat IL-13Ra2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu24-Lys336. |
Product Background |
Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2. |
Product Category |
Recombinant Protein |
Target |
IL-13Ra2 |
Gene/Protein ID |
Q8VHK6 |
Species |
Rat |
Exact Sequence |
Leu24-Lys336 |
Molecular Weight | The protein has a predicted MW of 63.17 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human IL-13, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat IL-13Ra2, hFc Tag with the EC50 of 10.7ng/ml determined by ELISA (QC Test). The affinity constant of 93.96 pM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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