• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: His16-Ala228
Catalog #ILR-RM122
Price
Regular price $33000
Regular price Sale price $33000
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Size
Avantor VWR Fisher Scientific

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Rat IL-22R alpha 1 Protein (ILR-RM122) Summary

Product Description

 Recombinant Rat IL-22R alpha 1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His16-Ala228.

Product Background

 Interleukin (IL)-22 is a member of the IL-10 family of cytokines and represents an important effector molecule of activated Th22, Th1, and Th17 cells, as well as Tc-cell subsets, gammadelta T cells, natural killer (NK), and NKT cells. IL-22 mediates its effects via a heterodimeric transmembrane receptor complex consisting of IL-22R1 and IL-10R2 and subsequent Janus kinase-signal transducers and activators of transcription (JAK-STAT) signaling pathways including Jak1, Tyk2, and STAT3.

Rat IL-22R alpha 1 Protein (ILR-RM122) Specifications/Details

Protein
 IL-22R alpha 1
Synonyms
 IL-22R-alpha-1; IL-22RA1; CRF2-9; IL22R; IL22R1; IL-TIF-R1; zcytoR11;IL22R-alpha-1; IL22RA1
Accession
Species
 Rat
Biotinylated
 no
Amino Acid Range
 His16-Ala228
Molecular Weight
 The protein has a predicted MW of 25.42 kDa. Due to glycosylation, the protein migrates to 37-45 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Rat IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 uM as determined in SPR assay (Biacore T200). Rat IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).
Rat IL-22R alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rat IL-22R alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat IL-22R alpha 1 is greater than 95% as determined by SEC-HPLC. The purity of Rat IL-22R alpha 1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...