SDS-PAGE gel showing strong single band for recombinant protein sample alongside molecular weight marker, indicating high expression level and purity
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Leu33-Leu342
  • Other Available Proteins:
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Catalog #LRG-RM101
Regular price $346.50
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Rat LRG1 Protein (LRG-RM101) Summary

Product Description

Recombinant Rat LRG1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu33-Leu342.

Product Background

Diabetic nephropathy (DN) is an important public health concern of increasing proportions and the leading cause of end-stage renal disease (ESRD) in diabetic patients. It is one of the most common long-term microvascular complications of diabetes mellitus that is characterized by proteinuria and glomerular structural changes. LRG1 is a novel pro-angiogenic factors involved in the abnormal angiogenesis and renal fibrosis in DN.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Rat LRG1 Protein (LRG-RM101) Specifications/Details

Protein
LRG1
Synonyms
Leucine-rich alpha-2-glycoprotein; LRG;LRG1
Accession
Species
Rat
Biotinylated
no
Amino Acid Range
Leu33-Leu342
Molecular Weight
The protein has a predicted MW of 35.6 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Rat LRG1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rat LRG1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat LRG1 is greater than 95% as determined by SEC-HPLC. The purity of Rat LRG1 is greater than 95% as determined by SEC-HPLC.
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