Product Description |
Recombinant Rat LRG1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu33-Leu342. |
Product Background |
Diabetic nephropathy (DN) is an important public health concern of increasing proportions and the leading cause of end-stage renal disease (ESRD) in diabetic patients. It is one of the most common long-term microvascular complications of diabetes mellitus that is characterized by proteinuria and glomerular structural changes. LRG1 is a novel pro-angiogenic factors involved in the abnormal angiogenesis and renal fibrosis in DN. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
LRG1 |
Synonyms |
Leucine-rich alpha-2-glycoprotein; LRG;LRG1 |
Accession |
|
Species |
Rat |
Biotinylated |
no |
Amino Acid Range |
Leu33-Leu342 |
Molecular Weight |
The protein has a predicted MW of 35.6 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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