Rat MASP2 Protein (MSP-RE102)

  • Express System: E.coli
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Rat MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Thr287-Phe685

Catalog: MSP-RE102

$405.00
Size:

Order now get it on

Product Description Recombinant Rat MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Thr287-Phe685.

Product Background

The dysregulation of complement cascade leads to unsolicited cytokine storm, inflammation, deterioration of alveolar lining cells, culminating in acquired respiratory destructive syndrome (ARDS). Similar pathogenesis is observed with the middle east respiratory syndrome (MERS), severe acquired respiratory syndrome (SARS), and SARS-CoV-2. Activation of the lectin pathway via mannose-binding lectin associated serine protease 2 (MASP2) is witnessed under discrete viral infections including COVID-19.

Product Category

Recombinant Protein

Target

MASP2
Synonyms MAP19; MASP-2; MASP1P1; sMap

Gene/Protein ID

A2VCV7

Species

Rat

Exact Sequence

Thr287-Phe685
Molecular Weight The protein has a predicted MW of 46.7 kDa. Due to autocatalytic cleavage, the protein migrates to 15-20 kDa&25-30 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Rat MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

E.coli
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in 50mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Rat MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.9ng/ml determined by ELISA.
Rat MASP2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in