Rat MASP2 Protein (MSP-RE102)
Rat MASP2 Protein (MSP-RE102)
Rat MASP2 Protein (MSP-RE102)
Rat MASP2 Protein (MSP-RE102)
  • Express System: E.coli
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Rat MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Thr287-Phe685
Catalog #MSP-RE102
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Product Description
 Recombinant Rat MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Thr287-Phe685.
Product Background
 The dysregulation of complement cascade leads to unsolicited cytokine storm, inflammation, deterioration of alveolar lining cells, culminating in acquired respiratory destructive syndrome (ARDS). Similar pathogenesis is observed with the middle east respiratory syndrome (MERS), severe acquired respiratory syndrome (SARS), and SARS-CoV-2. Activation of the lectin pathway via mannose-binding lectin associated serine protease 2 (MASP2) is witnessed under discrete viral infections including COVID-19.
Product Category
 Recombinant Protein
Target
 MASP2
Synonyms
 MAP19; MASP-2; MASP1P1; sMap
Accession
Species
 Rat
Amino Acid Range
 Thr287-Phe685
Molecular Weight
 The protein has a predicted MW of 46.7 kDa. Due to autocatalytic cleavage, the protein migrates to 15-20 kDa&25-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Rat MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 50mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Rat MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.9ng/ml determined by ELISA.
Rat MASP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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