Name: Rat MASP2 Protein (MSP-RE102)
Brand: KactusBio
SKU: MSP-RE102-100UG
Price: 445.5 USD
Availability: InStock

Express System: E.coli
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Rat MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.9ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Thr287-Phe685

Catalog #MSP-RE102

Size

100UG

500UG

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Product Description	Recombinant Rat MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Thr287-Phe685.
Product Background	The dysregulation of complement cascade leads to unsolicited cytokine storm, inflammation, deterioration of alveolar lining cells, culminating in acquired respiratory destructive syndrome (ARDS). Similar pathogenesis is observed with the middle east respiratory syndrome (MERS), severe acquired respiratory syndrome (SARS), and SARS-CoV-2. Activation of the lectin pathway via mannose-binding lectin associated serine protease 2 (MASP2) is witnessed under discrete viral infections including COVID-19.
Product Category	Recombinant Protein / Antibody discovery

Protein	MASP2
Synonyms	MAP19; MASP-2; MASP1P1; sMap
Accession	A2VCV7
Species	Rat
Biotinylated	no
Amino Acid Range	Thr287-Phe685
Molecular Weight	The protein has a predicted MW of 46.7 kDa. Due to autocatalytic cleavage, the protein migrates to 15-20 kDa&25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Rat MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Rat MASP2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.9ng/ml determined by ELISA.
	Rat MASP2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Rat MASP2 is greater than 95% as determined by SEC-HPLC.