Product Description |
Recombinant Rat PAI-1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser24-Pro402. |
Product Background |
PAI-1 (plasminogen activator inhibitor-1) is a member of plasminogen cascade with an inhibitory role in plasmin activation. PAI-1 is an important regulator of the fibrinolytic process and levels of this antifibrinolytic protein are elevated in diabetes and insulin resistant states. |
Product Category |
Recombinant Protein |
Target |
PAI-1 |
Synonyms |
PAI; PAI-1; Serpin E1; SERPINE1; PAI1; PLANH1 |
Accession |
|
Species |
Rat |
Biotinylated |
no |
Amino Acid Range |
Ser24-Pro402 |
Molecular Weight |
The protein has a predicted MW of 43.73 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50 mM HEPES, 300 mM NaCl, 1 mM TCEP (pH 7.5). Normally 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Rat PAI-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50-value is < 80-nM. |
|
|
The purity of Rat PAI-1 is greater than 95% as determined by SEC-HPLC. |
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