Name: Rat PAI-1 Protein (PAI-RM101)
Brand: KactusBio
SKU: PAI-RM101-100UG
Price: 264.0 USD
Availability: InStock

Catalog #PAI-RM101

Size

100UG

500UG

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Product Description	Recombinant Rat PAI-1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser24-Pro402.
Product Background	PAI-1 (plasminogen activator inhibitor-1) is a member of plasminogen cascade with an inhibitory role in plasmin activation. PAI-1 is an important regulator of the fibrinolytic process and levels of this antifibrinolytic protein are elevated in diabetes and insulin resistant states.
Product Category	Recombinant Protein / Antibody discovery

Protein	PAI-1
Synonyms	PAI; PAI-1; Serpin E1; SERPINE1; PAI1; PLANH1
Accession	P20961
Species	Rat
Biotinylated	no
Amino Acid Range	Ser24-Pro402
Molecular Weight	The protein has a predicted MW of 43.73 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50 mM HEPES, 300 mM NaCl, 1 mM TCEP (pH 7.5). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Rat PAI-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50-value is < 80-nM.
	The purity of Rat PAI-1 is greater than 95% as determined by SEC-HPLC.