Rat PAI-1 Protein (PAI-RM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Ser24-Pro402

Catalog: PAI-RM101

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Product Description Recombinant Rat PAI-1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser24-Pro402.

Product Background

PAI-1 (plasminogen activator inhibitor-1) is a member of plasminogen cascade with an inhibitory role in plasmin activation. PAI-1 is an important regulator of the fibrinolytic process and levels of this antifibrinolytic protein are elevated in diabetes and insulin resistant states.

Product Category

Recombinant Protein

Target

PAI-1

Gene/Protein ID

P20961

Species

Rat

Exact Sequence

Ser24-Pro402
Molecular Weight The protein has a predicted MW of 43.73 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in 50 mM HEPES, 300 mM NaCl, 1 mM TCEP (pH 7.5). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50-value is < 80-nM.
The purity of Rat PAI-1 is greater than 95% as determined by SEC-HPLC.
Rat PAI-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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