Rat PAI-1 Protein (PAI-RM101)
Rat PAI-1 Protein (PAI-RM101)
Rat PAI-1 Protein (PAI-RM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ser24-Pro402
Catalog #PAI-RM101
Regular price $264.00
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Product Description

Recombinant Rat PAI-1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser24-Pro402.

Product Background

PAI-1 (plasminogen activator inhibitor-1) is a member of plasminogen cascade with an inhibitory role in plasmin activation. PAI-1 is an important regulator of the fibrinolytic process and levels of this antifibrinolytic protein are elevated in diabetes and insulin resistant states.

Product Category

Recombinant Protein / Antibody discovery

Protein

PAI-1

Synonyms

PAI; PAI-1; Serpin E1; SERPINE1; PAI1; PLANH1

Accession

Species

Rat

Biotinylated

no

Amino Acid Range

Ser24-Pro402

Molecular Weight

The protein has a predicted MW of 43.73 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 50 mM HEPES, 300 mM NaCl, 1 mM TCEP (pH 7.5). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Rat PAI-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rat PAI-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50-value is < 80-nM. Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50-value is < 80-nM.
The purity of Rat PAI-1 is greater than 95% as determined by SEC-HPLC. The purity of Rat PAI-1 is greater than 95% as determined by SEC-HPLC.

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support@kactusbio.us

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