Rat PDGF-AA Protein (PGA-RY101)

  • Express System: Yeast
  • Product Tag: N-His
  • Purity: > 90% as determined by Tris-Bis PAGE
  • Amino Acid Range: Arg86-Lys204

Catalog: PGA-RY101

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Product Description Recombinant Rat PDGF-AA Protein is expressed from Yeast with His tag at the N-terminus.It contains Arg86-Lys204.

Product Background

PDGF (Platelet-derived growth factor) is one among numerous growth factors that regulate cell growth and division. The PDGF family forms five dimeric ligands, PDGF-AA, PDGF-BB, PDGF-CC, PDGF-DD, and PDGF-AB, which activate cognate receptor tyrosine kinases PDGFR-α and PDGFR-β. Upon binding to its tyrosine kinase receptor PDGF-αα, PDGF-AA stimulates the proliferation of osteoblastic cells and may exert autocrine and paracrine effects in regulating bone-forming processes. PDGF-AA is the most well characterized mitogen that is not restricted to any kind of tumor cell type but was seen in osteoblastic cells as well as in pleomorphic tumor elements.

Product Category

Recombinant Protein

Target

PDGF-AA
Accession

Species

Rat

Amino Acid Range

Arg86-Lys204
Molecular Weight The protein has a predicted MW of 14.75 kDa. Due to glycosylation, the protein migrates to 17-22 kDa and 25-45 kDa based on Tris-Bis PAGE result.

Product Tag

N-His

Express System

Yeast
Purity > 90% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 5 - 15 ng/mL.
Rat PDGF-AA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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