Rat PDGF-AA Protein (PGA-RY101) Summary
Product Description |
Recombinant Rat PDGF-AA Protein is expressed from Yeast with His tag at the N-terminus.It contains Arg86-Lys204. |
Product Background |
PDGF (Platelet-derived growth factor) is one among numerous growth factors that regulate cell growth and division. The PDGF family forms five dimeric ligands, PDGF-AA, PDGF-BB, PDGF-CC, PDGF-DD, and PDGF-AB, which activate cognate receptor tyrosine kinases PDGFR-α and PDGFR-β. Upon binding to its tyrosine kinase receptor PDGF-αα, PDGF-AA stimulates the proliferation of osteoblastic cells and may exert autocrine and paracrine effects in regulating bone-forming processes. PDGF-AA is the most well characterized mitogen that is not restricted to any kind of tumor cell type but was seen in osteoblastic cells as well as in pleomorphic tumor elements. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Rat PDGF-AA Protein (PGA-RY101) Specifications/Details
Protein |
PDGF-AA |
Synonyms |
PDGF-AA; PDGF-1; Pdgfa; PDGF-A; PDGF1; PDGF A; PDGFAA; PDGF AA; Rpa1 |
Accession |
|
Species |
Rat |
Biotinylated |
no |
Amino Acid Range |
Arg86-Lys204 |
Molecular Weight |
The protein has a predicted MW of 14.75 kDa. Due to glycosylation, the protein migrates to 17-22 kDa and 25-45 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Expression System |
Yeast |
Purity |
> 90% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Rat PDGF-AA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 5 - 15 ng/mL. |
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