Rat PDGF-AA Protein (PGA-RY101)
Rat PDGF-AA Protein (PGA-RY101)
Rat PDGF-AA Protein (PGA-RY101)
Rat PDGF-AA Protein (PGA-RY101)
  • Express System: Yeast
  • Product Tag: N-His
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Amino Acid Range: Arg86-Lys204
Catalog #PGA-RY101
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Product Description
 Recombinant Rat PDGF-AA Protein is expressed from Yeast with His tag at the N-terminus.It contains Arg86-Lys204.
Product Background
 PDGF (Platelet-derived growth factor) is one among numerous growth factors that regulate cell growth and division. The PDGF family forms five dimeric ligands, PDGF-AA, PDGF-BB, PDGF-CC, PDGF-DD, and PDGF-AB, which activate cognate receptor tyrosine kinases PDGFR-α and PDGFR-β. Upon binding to its tyrosine kinase receptor PDGF-αα, PDGF-AA stimulates the proliferation of osteoblastic cells and may exert autocrine and paracrine effects in regulating bone-forming processes. PDGF-AA is the most well characterized mitogen that is not restricted to any kind of tumor cell type but was seen in osteoblastic cells as well as in pleomorphic tumor elements.
Product Category
 Recombinant Protein
Target
 PDGF-AA
Accession
Species
 Rat
Biotinylated
 no
Amino Acid Range
 Arg86-Lys204
Molecular Weight
 The protein has a predicted MW of 14.75 kDa. Due to glycosylation, the protein migrates to 17-22 kDa and 25-45 kDa based on Bis-Tris PAGE result.
Product Tag
 N-His
Express System
 Yeast
Purity
 > 90% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Rat PDGF-AA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 5 - 15 ng/mL.
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