Rat PDGF R beta/CD140b Protein (PGF-RM1RB)
Rat PDGF R beta/CD140b Protein (PGF-RM1RB)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 67.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu32-Val531
Catalog #PGF-RM1RB
Regular price $379.50
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Product Description

Recombinant Rat PDGF R beta/CD140b Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu32-Val531.

Product Background

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

PDGF R beta/CD140b

Synonyms

JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; CD140B; PDGFR-2; PDGFR-β; PDGF R β

Accession

Species

Rat

Biotinylated

no

Amino Acid Range

Leu32-Val531

Molecular Weight

The protein has a predicted MW of 57.36 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.

Activity

The affinity constant of 67.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human PDGFB, His Tag immobilized on CM5 Chip can bind Rat PDGF R beta, His Tag with an affinity constant of 67.56 nM as determined in SPR assay (Biacore T200). Human PDGFB, His Tag immobilized on CM5 Chip can bind Rat PDGF R beta, His Tag with an affinity constant of 67.56 nM as determined in SPR assay (Biacore T200).
Rat PDGF R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rat PDGF R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat PDGF R beta is greater than 95% as determined by SEC-HPLC. The purity of Rat PDGF R beta is greater than 95% as determined by SEC-HPLC.

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