Rat PDGF R beta/CD140b Protein (PGF-RM1RB)
Rat PDGF R beta/CD140b Protein (PGF-RM1RB)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 67.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu32-Val531
Catalog #PGF-RM1RB
Regular price $345.00
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Product Description
Recombinant Rat PDGF R beta/CD140b Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu32-Val531.
Product Background
PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites.
Product Category
Recombinant Protein
Target
PDGF R beta/CD140b
Accession
Species
Rat
Biotinylated
no
Amino Acid Range
Leu32-Val531
Molecular Weight
The protein has a predicted MW of 57.36 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 67.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PDGFB, His Tag immobilized on CM5 Chip can bind Rat PDGF R beta, His Tag with an affinity constant of 67.56 nM as determined in SPR assay (Biacore T200).
Rat PDGF R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat PDGF R beta is greater than 95% as determined by SEC-HPLC.

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