Rat PDGF R beta/CD140b Protein (PGF-RM1RB)
Catalog: PGF-RM1RB
Order now get it on
Product Description | Recombinant Rat PDGF R beta/CD140b Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu32-Val531. |
Product Background |
PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. |
Product Category |
Recombinant Protein |
Target |
PDGF R beta/CD140b |
Gene/Protein ID |
Q05030 |
Species |
Rat |
Exact Sequence |
Leu32-Val531 |
Molecular Weight | The protein has a predicted MW of 57.36 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 67.56 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
You may also be interested in
View more associated products