Rat PLAU/uPA Protein (PLA-RM101)
Rat PLAU/uPA Protein (PLA-RM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: The affinity constant of 4.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly20-Phe432
Catalog #PLA-RM101
Regular price $315.00
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Product Description
Recombinant Rat PLAU/uPA Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly20-Phe432.
Product Background
Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.
Product Category
Recombinant Protein
Target
PLAU/uPA
Accession
Species
Rat
Biotinylated
no
Amino Acid Range
Gly20-Phe432
Molecular Weight
The protein has a predicted MW of 46.91 kDa. The protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 4.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human uPAR, hFc Tag captured on CM5 Chip via Protein A can bind Rat PLAU, His Tag with an affinity constant of 4.31 uM as determined in SPR assay (Biacore T200).
Rat PLAU on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat PLAU is greater than 90% as determined by SEC-HPLC.
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