Rat PLAU/uPA Protein (PLA-RM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Activity: The affinity constant of 4.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gly20-Phe432

Catalog: PLA-RM101

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Product Description	Recombinant Rat PLAU/uPA Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly20-Phe432.
Product Background	Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.
Product Category	Recombinant Protein

Target	PLAU/uPA
Gene/Protein ID	NP_037217.3
Species	Rat
Exact Sequence	Gly20-Phe432
Molecular Weight	The protein has a predicted MW of 46.91 kDa. The protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 4.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human uPAR, hFc Tag captured on CM5 Chip via Protein A can bind Rat PLAU, His Tag with an affinity constant of 4.31 uM as determined in SPR assay (Biacore T200).
	The purity of Rat PLAU is greater than 90% as determined by SEC-HPLC.
	Rat PLAU on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.