Rat PSMA/FOLH1 Protein (active dimer) (PSM-RM110)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >500 pmol/min/µg.
  • Amino Acid Range: Lys45-Asp752
  • Other Available Proteins:
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Catalog #PSM-RM110
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Size
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Rat PSMA/FOLH1 Protein (active dimer) (PSM-RM110) Summary

Product Description

 Recombinant Rat PSMA/FOLH1 Protein (active dimer) is expressed from HEK293 with His tag at the N-terminus.It contains Lys45-Asp752.

Product Background

 Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.

Product Category

 Recombinant Protein / Antibody discovery / CAR-T targets, ADC targets

Rat PSMA/FOLH1 Protein (active dimer) (PSM-RM110) Specifications/Details

Protein
 PSMA/FOLH1
Synonyms
 GCP2; PSMA; PSMAFGCP; FGCP; Folate hydrolase 1; FOLHNAALADase I; GCPII; GCPIImGCP; NAALAD1; NAALADase I; NAALAdase; FOLH1; GIG27; PSM; FOLH; mGCP
Accession
Species
 Rat
Biotinylated
 no
Amino Acid Range
 Lys45-Asp752
Molecular Weight
 The protein has a predicted MW of 80.62 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >500 pmol/min/µg.
Product Tag
 N-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 50mM MES, 150mM NaCl, 8% trehalose (pH 6.0).
Reconstitution
 Dissolve the lyophilized protein in 50mM MES (pH 6.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Rat PSMA on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Rat PSMA on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Rat PSMA is greater than 95% and the molecular weight of this protein is around 180-210 kDa as determined by SEC-MALS. The purity of Rat PSMA is greater than 95% and the molecular weight of this protein is around 180-210 kDa as determined by SEC-MALS.
The purity of Rat PSMA is greater than 95% as determined by SEC-HPLC. The purity of Rat PSMA is greater than 95% as determined by SEC-HPLC.
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