Rat Semaphorin 4A/SEMA4A Protein (SEM-RM14R)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Thr35-Ser680

Catalog #SEM-RM14R

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Rat Semaphorin 4A/SEMA4A Protein (SEM-RM14R) Summary

Product Description

Recombinant Rat Semaphorin 4A/SEMA4A Protein is expressed from HEK293 with His tag at the C-terminus.It contains Thr35-Ser680.

Product Background

Neuroimmune semaphorin 4A (Sema4A), a member of semaphorin family of transmembrane and secreted proteins, is an important regulator of neuronal and immune functions. In the nervous system, Sema4A primarily regulates the functional activity of neurons serving as an axon guidance molecule. In the immune system, Sema4A regulates immune cell activation and function, instructing a fine tuning of the immune response.

Rat Semaphorin 4A/SEMA4A Protein (SEM-RM14R) Specifications/Details

Protein	Semaphorin 4A/SEMA4A
Synonyms	Semaphorin-4A; Semaphorin-B; SEMA4A
Accession	F7F3I7
Species	Rat
Biotinylated	no
Amino Acid Range	Thr35-Ser680
Molecular Weight	The protein has a predicted MW of 72.57 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Rat Semaphorin 4A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Rat Semaphorin 4A is greater than 95% as determined by SEC-HPLC.