Rat TIGIT Protein (TIG-RM310)
  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse CD155, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat TIGIT, mFc Tag with the EC50 of 23.1ng/ml determined by ELISA.
  • Amino Acid Range: Ala17-Gln136
Catalog #TIG-RM310
Regular price $347.00
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Size
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Rat TIGIT Protein (TIG-RM310) Summary

Product Description

Recombinant Rat TIGIT Protein is expressed from HEK293 with mFc (IgG2a) tag at the C-terminus.It contains Ala17-Gln136.

Product Background

TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Rat TIGIT Protein (TIG-RM310) Specifications/Details

Protein
TIGIT
Synonyms
TIGIT; VSIG9; VSTM3; WUCAM
Accession
Species
Rat
Biotinylated
no
Amino Acid Range
Ala17-Gln136
Molecular Weight
The protein has a predicted MW of 39.47 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse CD155, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat TIGIT, mFc Tag with the EC50 of 23.1ng/ml determined by ELISA.
Product Tag
C-mFc

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse CD155, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat TIGIT, mFc Tag with the EC50 of 23.1ng/ml determined by ELISA. Immobilized Mouse CD155, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Rat TIGIT, mFc Tag with the EC50 of 23.1ng/ml determined by ELISA.
Rat TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rat TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Mouse CD155, His Tag on Anti-His-Biosensor can bind Rat TIGIT, mFc Tag with an affinity constant of 6.93 nM as determined in BLI assay . Loaded Mouse CD155, His Tag on Anti-His-Biosensor can bind Rat TIGIT, mFc Tag with an affinity constant of 6.93 nM as determined in BLI assay .
The purity of Rat TIGIT is greater than 95% as determined by SEC-HPLC. The purity of Rat TIGIT is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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